Products

Induced Pluripotent Stem Cells (iPSCs) Cryopreservation Solution

A DMSO-free cryopreservation solution tailored specifically for iPSC cells, contains combinations of sugars, sugar alcohols, and amino acids in the optimal ratios to protect cells. The solutions are available to order, to be developed fit-for-purpose or through collaborative research.

Advantages of DMSO-free solution

Our solution alleviates pain points associated with the use of DMSO in the cryopreservation of human Induced Pluripotent Stem cells. Both hiPSCs and cells differentiated from them are commonly multicellular systems, which are more sensitive to stresses of freezing and thawing than single cells.

Research conducted demonstrated the ability to replace DMSO with non-toxic molecules, improve post-thaw cell survival, and reduce sensitivity to undercooling.

Results of additional comparative studies against leading commercialized DMSO-based products reveal nearly 100% cell viability in comparison to 20-30% with currently available options, for a 4-fold improvement.

Mesenchymal Stromal Cells (MSCs) Cryopreservation Solution

A DMSO-free cryopreservation solution tailored specifically for MSC, contains combinations of sugars, sugar alcohols, and amino acids in the optimal ratios to protect cells. The solutions are available off the shelf, fit-for-purpose or through collaborative research.

Advantages of DMSO-free solution

Our solution alleviates pain points associated with the use of DMSO in the cryopreservation of human MSCs. Both MSCs and cells differentiated from them are commonly multicellular systems, which are more sensitive to stresses of freezing and thawing than single cells.

Research conducted demonstrated the ability to replace DMSO with non-toxic molecules, improve post-thaw cell survival, and reduce sensitivity to undercooling.

Results of additional comparative studies against leading commercialized DMSO-based products reveal nearly 100% cell viability.


Post Thaw Cell Viability

Multivariable research demonstrated that our osmolyte-based solution does not exhibit the same sensitivity to undercooling as those frozen in DMSO-based solution, indicating superior adaptability of the optimized solutions to different freezing modalities and variability in methodology.

As hiPSCs can differentiate into all cell types, high efficiency storage and manufacturing is critical for downstream clinical and commercial production.

Efficacy and proof of concept research depends on consistent, reproducible results with high cell viability and functionality in early stage research. By eliminating the fluctuation and low outcomes of DMSO-based cryopreservation early in research stages, researchers are able to focus on optimizing the valid potential of the iPSC study.


Publications

Overview
  1. Hubel, A. Impact of freeze-thaw processes on the quality of cells. BioInsights. 12, 2017. 807-813.
Immunotherapies
  1. Li, R et al. Preservation of cell-based immunotherapies for clinical trials. Cytotherapy. 2019; 21. 943-957.
  2. Yu, G and Hubel, A. The Role of Preservation in the Variability of Regenerative Medicine Products. Regenerative Engineering and Translational Medicine. 2019. 1-9

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